Product Pathways - Chromatin Regulation
Acetyl-Histone H2B (Lys20) Antibody #2571
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-IC IC | H M R | Endogenous | 14 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
IC=Immunocytochemistry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Acetyl-Histone H2B (Lys20) Antibody detects endogenous levels of histone H2B only when acetylated at lysine 20. The antibody does not cross-react with other acetylated histones.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic acetylated peptide (KLH-coupled) corresponding to sequence surrounding Lys20 of human histone H2B. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, untreated or TSA-treated (400 nM for 12 hours), using Acetyl-Histone H2B (Lys20) Antibody.
IHC-P (paraffin)
Immunohistochemical staining of paraffin-embedded human breast carcinoma, showing nuclear localization of histone H2B, using Acetyl-Histone H2B (Lys20) Antibody (left), or the same antibody preincubated with specific acetyl histone H2B peptide (right).
IC-ABC
Immunocytochemical staining of NIH/3T3 cells, untreated or TSA-treated (400 nM for 12 hours), using Acetyl-Histone H2B (Lys20) Antibody.
IF-IC
Immunocytochemical staining of Arabidopsis root cells showing nuclear localization, using Acetyl-Histone H2B (Lys20) Antibody. (Kindly provided by Dr. M.K. Kandasamy, University of Georgia.)
Background
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11).
- Workman, J.L. and Kingston, R.E. (1998) Annu. Rev. Biochem. 67, 545-579.
- Hansen, J.C. et al. (1998) Biochemistry 37, 17637-17641.
- Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-45.
- Cheung, P. et al. (2000) Cell 103, 263-271.
- Bernstein, B.E. and Schreiber, S.L. (2002) Chem. Biol. 9, 1167-1173.
- Jaskelioff, M. and Peterson, C.L. (2003) Nat. Cell Biol. 5, 395-399.
- Thorne, A.W. et al. (1990) Eur. J. Biochem. 193, 701-713.
- Hendzel, M.J. et al. (1997) Chromosoma 106, 348-360.
- Goto, H. et al. (1999) J. Biol. Chem. 274, 25543-25549.
- Preuss, U. et al. (2003) Nucleic Acids Res. 31, 878-885.
- Dai, J. et al. (2005) Genes Dev. 19, 472-488.
Application References
- Parsons, X. H. et al. (2003) Histone deacetylation by Sir2 generates a transcriptionally repressed nucleoprotein complex. Proc. Nat. Acad. Sci. USA 100, 1609-1614. This article references the use of Acetyl-Histone H2B (Lys20) Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9671 Acetyl-Histone H3 (Lys9) Antibody
- 9674 Acetyl-Histone H3 (Lys23) Antibody
- 9701 Phospho-Histone H3 (Ser10) Antibody
- 9706 Phospho-Histone H3 (Ser10) (6G3) Mouse mAb
- 9711 Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody
- 9715 Histone H3 Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2722 Histone H2B Antibody
- 2592 Histone H4 Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
