Product Pathways - Tyrosine Kinase / Adaptors
Heregulin Antibody #2573
PhosphoSitePlus® protein, site, and accession data: NRG1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M | Endogenous | 105 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2573:
- Western Blotting
Specificity / Sensitivity
Heregulin Antibody detects endogenous levels of total heregulin proteins. The antibody does not cross-react with other family members.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His494 of human heregulin. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from various cell lines using Heregulin Antibody.
Background
Heregulin (HRG), also called neuregulin (NRG1), neu differentiation factor (NDF) or glial growth factor-2 (GGF-2), is a soluble growth factor synthesized as a transmembrane precursor molecule. Metalloproteinases and other proteases catalyze the cleavage of its extracellular domain which is then released and functions as a ligand for ErbB3 and ErbB4 receptor tyrosine kinase. The signaling pathways of HRG-ErbB3/ErbB4 are involved in regulation of cell proliferation, differentiation, invasion, and survival of both normal and malignant tissues (1,2). Abnormality of HRG-ErbB signaling leads to development of a variety of human diseases.HRG family has four isoforms including HRG-1, -2, -3 and -4, which are derived from alternative exon splicing. Moreover, they showed various tissue expression and biological activities (3).
- Breuleux, M. (2007) Cell Mol Life Sci 64, 2358-77.
- Ozaki, M. et al. (2004) J Neurochem 91, 176-88.
- Falls, D.L. (2003) Exp Cell Res 284, 14-30.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
