Product Pathways - Translational Control
Ribosomal Protein S3 Antibody #2579
Have you tried your application using our XP® monoclonal antibodies? Try product: 9538
PhosphoSitePlus® protein, site, and accession data: RPS3
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R Mk | Endogenous | 27 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Ribosomal Protein S3 Antibody detects endogenous levels of total ribosomal protein S3.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ribosomal protein S3. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using Ribosomal Protein S3 Antibody.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Ribosomal Protein S3 Antibody (green). Actin filamens have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Ribosomal protein S3 (rpS3) is a component of the 40S ribosomal subunit and is involved in translation. HSP90 interacts with both the amino-terminus and carboxy-terminus of rpS3, preventing its ubiquitination and degradation and thereby retaining the integrity of the ribosome (1). rpS3 has also been shown to function as an endonuclease during DNA damage repair (2,3). Furthermore, overexpression of rpS3 sensitizes lymphocytic cells to cytokine-induced apoptosis, indicating a third role for rpS3 during apoptosis (4). The functions of rpS3 during DNA damage repair and apoptosis have been mapped to two distinct domains (4).
- Kim, T.S. et al. (2006) Mol Biol Cell 17, 824-33.
- Kim, J. et al. (1995) J Biol Chem 270, 13620-9.
- Choi, S.H. et al. (2006) FEBS Lett 580, 6755-62.
- Jang, C.Y. et al. (2004) FEBS Lett 560, 81-5.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
