Cell Signaling Technology

Product Pathways - Phosphatases

PP1α Antibody #2582

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Dm Endogenous 38 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dm=D. melanogaster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PP1α Antibody detects endogenous levels of total PP1α protein. This antibody does not cross-react with PP1δ or PP1γ.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human PP1alpha. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of purified recombinant PP1α protein (lane 1), and extracts from C6, NIH/3T3 and HeLa cells (lanes 2-4), using PP1α Antibody.

Background

Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4).

  1. Rubin, E. et al. (1998) Front. Biosci. 3, D1209-1219.
  2. Durfee, T. et al. (1993) Genes Dev. 7, 555-569.
  3. Izumi, T. et al. (1992) Mol. Cell. Biol. 3, 927-939.
  4. Kwon, Y. G. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 2168-2173.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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