Cell Signaling Technology

Product Pathways - Phosphatases

PP1alpha Antibody #2582

Applications Reactivity MW (kDa) Source
W H M R Mk Dr 38 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dr=Drosophila
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

PP1alpha Antibody detects endogenous levels of total PP1alpha protein. This antibody does not cross-react with PP1delta or PP1gamma.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the amino-terminal sequence of human PP1alpha. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of purified recombinant PP1alpha protein (lane 1), and extracts from C6, NIH/3T3 and HeLa cells (lanes 2-4), using PP1alpha Antibody.

Background

Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4).

  1. Rubin, E. et al. (1998) Front. Biosci. 3, D1209-1219.
  2. Durfee, T. et al. (1993) Genes Dev. 7, 555-569.
  3. Izumi, T. et al. (1992) Mol. Cell. Biol. 3, 927-939.
  4. Kwon, Y. G. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 2168-2173.

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