Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

PCNA (PC10) Mouse mAb #2586

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC H M R Mk B Pg Endogenous 36 Mouse IgG2a

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

PCNA (PC10) Mouse mAb detects endogenous levels of PCNA protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein obtained from pC2T.

Western Blotting

Western Blotting

Western blot analysis of human (HeLa), murine (SV-T2), bovine (BAEC), and porcine (PAE) cell extracts using PCNA (PC10) Mouse mAb.

IP

IP

Western blot analysis, using PCNA (PC10) Mouse mAb (upper) and Beta-Actin Antibody #4967(lower), of HeLa cell extract (lane 1) and PCNA immunoprecipitated from the same extract using PCNA (PC10) Mouse mAb (lane 2).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NCI-H460 cells using PCNA (PC10) Mouse mAb (green), Phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb (Alexa Fluor 555 Conjugate) (red), and p21 Waf1/Cip1 (12D1) Rabbit mAb (blue).


Background

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.

  1. Kelman, Z. and O'Donnell, M. (1995) Nucleic Acids Res. 23, 3613-3620.
  2. Krishna, T. S. et al. (1994) Cell 79, 1233-1243.
  3. Maga, G. and Hubscher, U. (2003) J. Cell Sci. 116, 3051-3060.
  4. Sawey, E.T. et al. (2011) Cancer Cell 19, 347-58.

Application References

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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