Product Pathways - Cell Cycle / Checkpoint
PCNA (PC10) Mouse mAb #2586
PhosphoSitePlus® protein, site, and accession data: PCNA
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M R Mk B Pg | Endogenous | 36 | Mouse IgG2a |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
* Product-specific protocol.
Specificity / Sensitivity
PCNA (PC10) Mouse mAb detects endogenous levels of PCNA protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein obtained from pC2T.
Western Blotting
Western blot analysis of human (HeLa), murine (SV-T2), bovine (BAEC), and porcine (PAE) cell extracts using PCNA (PC10) Mouse mAb.
IP
Western blot analysis, using PCNA (PC10) Mouse mAb (upper) and Beta-Actin Antibody #4967(lower), of HeLa cell extract (lane 1) and PCNA immunoprecipitated from the same extract using PCNA (PC10) Mouse mAb (lane 2).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using PCNA (PC10) Mouse mAb and propidium iodide (DNA content). Propidium Iodide (PI)/RNase Staining Solution #4087. Anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.
IF-IC
Confocal immunofluorescent analysis of NCI-H460 cells using PCNA (PC10) Mouse mAb (green), Phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb (Alexa Fluor 555 Conjugate) (red), and p21 Waf1/Cip1 (12D1) Rabbit mAb (blue).
Background
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.
- Kelman, Z. and O'Donnell, M. (1995) Nucleic Acids Res. 23, 3613-3620.
- Krishna, T. S. et al. (1994) Cell 79, 1233-1243.
- Maga, G. and Hubscher, U. (2003) J. Cell Sci. 116, 3051-3060.
- Sawey, E.T. et al. (2011) Cancer Cell 19, 347-58.
Application References
- Forrester, M.T. et al. (2011) Biotechniques 50, 41-5. Applications: Western Blotting
- Han, B. et al. (2011) J Immunol 186, 5833-44. Applications: IF-IC (In Cells)
- Desai, S. et al. (2010) Mol Cancer Res 8, 907-18. Applications: IF-IC (In Cells)
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
