Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

DLL1 Antibody #2588

Applications Reactivity Sensitivity MW (kDa) Source
W IP R (H) Transfected Only 82 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  R=Rat
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

DLL1 Antibody detects transfected levels of DLL1. It does not recognize transfected levels of rat DLL3 and human DLL4.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region surrounding residue Ala627 of human DLL1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transiently transfected with a construct expressing rat DLL1 protein, using DLL1 Antibody.

Background

Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6).

  1. Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868.
  2. Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328.
  3. Chiba, S. (2006) Stem Cells 24, 2437-2447.
  4. Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610.
  5. Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643.
  6. LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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