Cell Signaling Technology

Product Pathways - Chromatin Regulation

SirT6 Antibody #2590

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H Endogenous 42 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

SirT6 Antibody detects endogenous levels of total SirT6 protein. This antibody does not cross-react with other sirtuin proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of the human SirT6 protein. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of various cell types using SirT6 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using SirT6 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Background

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway (2-4). The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H2O2 (2). In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres and gaps (2). SirT6 may regulate the BER pathway by deacetylating DNA Polβ or other core components of the pathway (2).

  1. Guarente, L. (1999) Nat Genet 23, 281-5.
  2. Mostoslavsky, R. et al. (2006) Cell 124, 315-29.
  3. Liszt, G. et al. (2005) J Biol Chem 280, 21313-20.
  4. Michishita, E. et al. (2005) Mol Biol Cell 16, 4623-35.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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