Product Pathways - Lymphocyte Signaling
Evi-1 (C50E12) Rabbit mAb #2593
|2593S||100 µl (10 western blots)||---||In Stock||---|
|2593||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human||Endogenous||105, 145, 200||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
Evi-1 (C50E12) Rabbit mAb detects the endogenous levels of total Evi-1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu56 of human Evi-1.
Western blot analysis of extracts from various cell lines using Evi-1 (C50E12) Rabbit mAb. MDS/Evi-1 denotes the fusion between MDS1 and Evi-1 (see reference #6) and Evi-1 Δ denotes the truncated form of Evi-1 (see reference #5).
Flow cytometric analysis of SK-OV-3 cells using Evi-1 (C50E12) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Confocal immunofluorescent analysis of SK-OV-3 (left) and DLD-1 cells (right) using Evi-1 (C50E12) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Evi-1 (Ecotropic virus integration site 1) was originally identified as a common site of viral integration in murine myeloid leukemia. It is involved in human myeloid disorders through chromosome translocation and inversion (1) and is also implicated in solid tumor formation (2). Evi-1 is a zinc finger transcription factor which also plays an important role in animal development (3). It has many isoforms due to alternative usage of 5'-ends (4), alternative splicing (5), and intergenic splicing which results in the formation of a fusion protein with MDS1 in normal tissues (6).
- Goyama, S. et al. (2008) Cell Stem Cell 3, 207-20. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.