Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Evi-1 (C50E12) Rabbit mAb #2593

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H Endogenous 105, 145, 200 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Evi-1 (C50E12) Rabbit mAb detects the endogenous levels of total Evi-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu56 of human Evi-1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Evi-1 (C50E12) Rabbit mAb. MDS/Evi-1 denotes the fusion between MDS1 and Evi-1 (see reference #6) and Evi-1 Δ denotes the truncated form of Evi-1 (see reference #5).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SK-OV-3 cells using Evi-1 (C50E12) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of SK-OV-3 (left) and DLD-1 cells (right) using Evi-1 (C50E12) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).


Background

Evi-1 (Ecotropic virus integration site 1) was originally identified as a common site of viral integration in murine myeloid leukemia. It is involved in human myeloid disorders through chromosome translocation and inversion (1) and is also implicated in solid tumor formation (2). Evi-1 is a zinc finger transcription factor which also plays an important role in animal development (3). It has many isoforms due to alternative usage of 5'-ends (4), alternative splicing (5), and intergenic splicing which results in the formation of a fusion protein with MDS1 in normal tissues (6).

  1. Nucifora, G. et al. (2006) Gene 368, 1-11.
  2. Brooks, D.J. et al. (1996) Br. J. Cancer 74, 1518-25.
  3. Hoyt, P.R. et al. (1997) Mech. Dev. 65, 55-70.
  4. Aytekin, M. et al. (2005) Gene 356, 160-8.
  5. Morishita, K. et al. (1990) Oncogene 5, 963-71.
  6. Fears, S. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 1642-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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