Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

Naked2 (C36D3) Rabbit mAb #2596

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP M Transfected Only 59, 61 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Naked2 (C36D3) Rabbit mAb detects transfected levels of mouse Naked2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly47 of mouse Naked2.

Western Blotting

Western Blotting

Western blot analysis of COS cell extracts, untransfected or transiently transfected with a construct expressing mouse Naked2, using Naked2 (C36D3) Rabbit mAb.

Background

Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif, however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/β-catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF-α to the basolateral plasma membrane of polarized epithelial cells (6).

  1. Zeng, W. et al. (2000) Nature 403, 789-795.
  2. Wharton, K.A. et al. (2001) Dev. Biol. 234, 93-106.
  3. Rousset, R. et al. (2002) J. Biol. Chem. 277, 49019-49026.
  4. Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 3802-3807.
  5. Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14973-14978.
  6. Li, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 5571-5576.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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