Product Pathways - Cytoskeletal Signaling
NUP98 (C39A3) Rabbit mAb #2598
PhosphoSitePlus® protein, site, and accession data: NUP98
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H M R Mk | Endogenous | 98 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
NUP98 (C39A3) Rabbit mAb detects endogenous levels of total NUP98 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro671 of human NUP98.
Western Blotting
Western blot analysis of cell extracts from various cell types using NUP98 (C39A3) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of Caki-1 cells using NUP98 (C39A3) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Background
NUP98 (nucleoporin 98 kDa) is a component of the nuclear pore complex. It is expressed as three different precursors that undergo auto-cleavage to generate a common amino-terminal 98 kDa peptide (NUP98) and carboxy-terminal 6, 96 (NUP96) and 88 (p88) kDa peptides (1-2). NUP98 contains FG and GLFG repeat domains at its amino terminus and a RNA-binding domain in its carboxy terminus (3). The NUP98 gene is localized on chromosome 11p15.5, a region frequently rearranged in leukemias. To date, 15 fusion partners have been identified for NUP98 (4-5).
- Fontoura, B.M. et al. (1999) J. Cell Biol. 144, 1097-1112.
- Rosenblum, J.S. and Blobel, G. (1999) Proc. Natl. Acad. Sci. USA 96, 11370-11375.
- Griffis, E.R. et al. (2004) Mol. Biol. Cell 15, 1991-2002.
- Slape, C. and Aplan, P.D. (2004) Leuk. Lymphoma 45, 1341-1350.
- Nakamura, T. (2005) Int. J. Hematol. 82, 21-27.
Application References
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Companion Products
- 2288 NUP98 (L205) Antibody
- 2292 NUP98 (P671) Antibody
- 2597 NUP98 (C37G10) Rabbit mAb
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
