Product Pathways - Translational Control
PRAS40 Antibody #2610
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 40 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
PRAS40 Antibody detects endogenous levels of total PRAS40 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human PRAS40. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using PRAS40 Antibody.
Background
Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4).
- Cantley, L.C. (2002) Science 296, 1655-7.
- Kovacina, K.S. et al. (2003) J Biol Chem 278, 10189-94.
- Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23.
- Sancak, Y. et al. (2007) Mol Cell 25, 903-15.
Application References
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Companion Products
- 2691 PRAS40 (D23C7) Rabbit mAb
- 2640 Phospho-PRAS40 (Thr246) Antibody
- 2983 mTOR (7C10) Rabbit mAb
- 2972 mTOR Antibody
- 2280 Raptor (24C12) Rabbit mAb
- 2083 Phospho-Raptor (Ser792) Antibody
- 3274 GβL (86B8) Rabbit mAb
- 3227 GβL Antibody
- 2140 Rictor Antibody
- 2114 Rictor (53A2) Rabbit mAb
- 2971 Phospho-mTOR (Ser2448) Antibody
- 2974 Phospho-mTOR (Ser2481) Antibody
- 2976 Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific)
- 9964 mTOR Pathway Antibody Sampler Kit
- 9997 Tris Buffered Saline with Tween 20 (TBST - 10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
