Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody #2611

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 120 Phospho-PRK1. 140 Phospho-PRK2. Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-PRK1 (Thr774)/ PRK2 (Thr816) Antibody detects endogenous levels of PRK1 and PRK2 only when phosphorylated at Thr774 or Thr816, respectively. This antibody also detects PKC zeta and lambda when phosphorylated at Thr 410 and 403, respectively.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr774 of human PRK1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or calf intestinal alkaline phosphotase (CIP)-treated, and 293 cells, untreated or calyculin A-treated, using Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody (upper) or PRK1/PRK2 antibody (lower).

Background

The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids in vitro (7,8). Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10).

  1. Mukai, H. et al. (1994) Biochem. Biophys. Res. Commun. 199, 897-904.
  2. Morrice, N.A. et al. (1994) J. Biol. Chem. 269, 20040-20046.
  3. Palmer, R.H. et al. (1994) FEBS Lett. 356, 5-8.
  4. Watanabe, G. et al. (1996) Science 271, 645-648.
  5. Amano, M. et al. (1996) Science 271, 648-650.
  6. Vincent, S. and Settleman, J. (1997) Mol. Cell. Biol. 17, 2247-2256.
  7. Morrice, N.A. et al. (1994) FEBS Lett. 351, 171-175.
  8. Palmer, R.H. et al. (1995) J. Biol. Chem. 270, 22412-22416.
  9. Flynn, P. et al. (2000) J. Biol. Chem. 275, 11064-70.
  10. Dong, L.Q. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5089-94.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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