Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

PRK2 Antibody #2612

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 140 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PRK2 Antibody detects endogenous levels of total PRK2 protein. The antibody does not cross-react with PRK1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human PRK2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using PRK2 Antibody.

Background

The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids in vitro (7,8). Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10).

  1. Mukai, H. et al. (1994) Biochem. Biophys. Res. Commun. 199, 897-904.
  2. Morrice, N.A. et al. (1994) J. Biol. Chem. 269, 20040-20046.
  3. Palmer, R.H. et al. (1994) FEBS Lett. 356, 5-8.
  4. Watanabe, G. et al. (1996) Science 271, 645-648.
  5. Amano, M. et al. (1996) Science 271, 648-650.
  6. Vincent, S. and Settleman, J. (1997) Mol. Cell. Biol. 17, 2247-2256.
  7. Morrice, N.A. et al. (1994) FEBS Lett. 351, 171-175.
  8. Palmer, R.H. et al. (1995) J. Biol. Chem. 270, 22412-22416.
  9. Flynn, P. et al. (2000) J. Biol. Chem. 275, 11064-70.
  10. Dong, L.Q. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5089-94.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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