Product Pathways - Phosphatases
Pan-Calcineurin A Antibody #2614
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC F | H M R Mk Dm (C) (X) (B) (Pg) | Endogenous | 59 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
C=Chicken
Dm=D. melanogaster
X=Xenopus
B=Bovine
Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
This antibody detects endogenous levels of Calcineurin A (alpha isoform). It may also recognize the beta and gamma isoforms of Calcineurin A. The antibody does not cross-react with protein phosphatase 1 or 2A.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human Calcineurin A (alpha isoform). Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of lysates from MCF7, NIH/3T3, C6 and COS cells, using Pan-Calcineurin A antibody.
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells, using Pan-Calcineurin A antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of Hela cells, using Pan-Calcineurin A Antibody exhibiting cytoplasmic localization (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Calcineurin, also known as protein phosphatase 2B (PP2B), is a calmodulin-dependent, calcium-activated, serine/threonine protein phosphatase composed of a catalytic subunit (calcineurin A) and a tightly bound regulatory subunit (calcineurin B) (1). Calcineurin A is highly homologous to protein phosphatases 1 and 2A. Calcineurin B, like calmodulin, contains four EF-hand, calcium-binding motifs.Calcineurin signaling has been implicated in a broad spectrum of cellular processes including cell-cycle regulation, stress response and apoptosis and is required for proper cardiovascular and skeletal muscle development (2,3). Calcineurin-mediated dephosphorylation of the nuclear factor of activated T cells (NFAT) transcription factor is essential for NFAT activation and nuclear translocation and early gene expression in T lymphocytes (2,3). Calcineurin is the target of the immunosuppressive drugs Cyclosporin A and FK506, both of which block the activation of quiescent T cells after T cell receptor engagement (2,3). Cyclosporin A and FK506 bind to the immunophilins, cyclophilin and FKBP respectively and the immunophilin-drug complex binds to calcineurin and blocks substrate binding.
- Rusnak, F. and Mertz, P. (2000) Physiol. Rev. 80, 1483-521.
- Kahl, C.R. and Means, A.R. (2003) Endocr. Rev. 24, 719-36.
- Schulz, R.A. and Yutzey, K.E. (2004) Dev. Biol. 266, 1-16.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
