Product Pathways - Translational Control
eIF4G (C65H5) Rabbit mAb #2617
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W | H M R Mk | Endogenous | 220 | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
eIF4G (C65H5) Rabbit mAb detects endogenous levels of total eIF4G protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence around Ala881 of human eIF4G.
Western Blotting
Western blot analysis of extracts from PANC1 and NIH/3T3 cells using eIF4G (C65H5) Rabbit mAb.
Background
The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5' m7GTP mRNA cap and function to unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A (4) and serves as an adaptor molecule in the eIF4 complex. Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). Two distinct eIF4G species have been identified; eIF4GI and eIF4GII share 46% identity in their amino acid sequences and are functionally related homologs (7).
- Yan, R. and Rhoads, R.E. (1995) Genomics 26, 394-398.
- Morley, S.J. et al. (1997) RNA 3, 1085-1104.
- Haghighat, A. and Sonenberg, N. (1997) J. Biol. Chem. 272, 21677-21680.
- De Gregorio, E. et al. (1998) RNA 4, 828-836.
- Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
- Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
- Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2441 Phospho-eIF4G (Ser1108) Antibody
- 2498 eIF4G Antibody
- 2469 eIF4G (C45A4) Rabbit mAb
- 2490 eIF4A1 Antibody
- 9741 Phospho-eIF4E (Ser209) Antibody
- 9742 eIF4E Antibody
- 4992 PABP1 Antibody
- 9997 Tris Buffered Saline with Tween 20 (TBST - 10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
