Product Pathways - Chromatin Regulation

JMJD1B/JHDM2B Antibody #2621

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP IF-IC	H M R Mk	Endogenous	220	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

JMJD1B/JHDM2B Antibody detects endogenous levels of JMJD1B protein (all three isoforms).

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the human JMJD1B protein. Antibodies are purified by peptide affinity chromatography.

Background

The methylation state of lysine residues in histone proteins is a major determinant for formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JHDM2A, JMJD1B/JHDM2B, JMJD1C/JHDM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen receptor and thyroid receptor and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8).

1. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
2. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
3. Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27.
4. Cachon-Gonzalez, M.B. et al. (1994) Proc Natl Acad Sci USA 91, 7717-21.
5. Ahmad, W. et al. (1998) Science 279, 720-4.
6. Potter, G.B. et al. (2001) Genes Dev 15, 2687-701.
7. Hoog, C. et al. (1991) Mol Reprod Dev 30, 173-81.
8. Yamane, K. et al. (2006) Cell 125, 483-95.
9. Okada, Y. et al. (2007) Nature 450, 119-23.
10. Loh, Y.H. et al. (2007) Genes Dev 21, 2545-57.
11. Ko, S.Y. et al. (2006) Cell Struct Funct 31, 53-62.
12. Hu, Z. et al. (2001) Oncogene 20, 6946-54.
13. Lee, J.W. et al. (1995) Mol Endocrinol 9, 243-54.
14. Wolf, S.S. et al. (2007) Arch Biochem Biophys 460, 56-66.
15. Castermans, D. et al. (2007) Eur J Hum Genet 15, 422-31.

Application References

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Companion Products

• 2184 LSD1 (C69G12) Rabbit mAb
• 2139 LSD1 Antibody
• 4069 Pan-Methyl-Histone H3 (Lys9) Antibody
• 9753 Di-Methyl-Histone H3 (Lys9) Antibody
• 9715 Histone H3 Antibody
• 3202 Androgen Receptor Antibody
• 2750 Oct-4 Antibody
• 9139 Stat3 (124H6) Mouse mAb

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.