Product Pathways - Complementary Reagents

GST Antibody #2622

Applications	Reactivity	Sensitivity	Source
W IP	All	Transfected Only	Rabbit

Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

2622:: Immunoprecipitation, Western Blotting

Specificity / Sensitivity

GST Antibody detects transfected GST fusion proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a GST fusion protein. Antibodies are purified by protein A and GST affinity chromatography.

Western Blotting

Western blot analysis of extracts from untransfected control cells (-) and transfected cells overexpressing GST-Bad (+), using GST Antibody (left) and Bad Antibody #9292 (right).

Western Blotting

Western blot analysis of purified recombinant GST fusion proteins.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).

Application References

Widenmaier, S.B. et al. (2009) J Biol Chem 284, 30372-82. Applications: Western Blotting

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.