GST (26H1) Mouse mAb #2624
|2624S||100 µl (10 western blots)||---||In Stock||---|
|2624||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Transfected Only||Mouse IgG2a|
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
GST (26H1) Mouse mAb detects transfected glutathione S-transferase (GST) fusion proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein.
Western blot analysis of extracts from transfected COS-7 cells overexpressing GST-Bad and untransfected control, using GST (26H1) Mouse mAb (left) or anti-Bad Antibody (right).
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
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