Product Pathways - Companion Products
GST (91G1) Rabbit mAb #2625
|W IP IF-IC||All||Transfected Only||Rabbit IgG|
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Specificity / Sensitivity
GST (91G1) Rabbit mAb detects transfected GST fusion proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein.
Western blot analysis of extracts from untransfected control cells (-) and transfected cells overexpressing GST-Bad (+), using GST (91G1) Rabbit mAb (left) and Bad Antibody #9292 (right).
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
- Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
- Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
- Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.
- Yamada, M. et al. (2006) J Biol Chem 281, 20749-60. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.