GST (91G1) Rabbit mAb #2625
|2625S||100 µl (10 western blots)||---||In Stock||---|
|2625P||40 µl (4 western blots)||---||In Stock||---|
|2625||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Transfected Only||Rabbit IgG|
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
GST (91G1) Rabbit mAb detects transfected GST fusion proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein.
Western blot analysis of extracts from COS-7 cells untransfected (-) and transfected cells overexpressing GST-Bad (+), using GST (91G1) Rabbit mAb (left) and Bad Antibody #9292 (right).
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
- Yamada, M. et al. (2006) J Biol Chem 281, 20749-60. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.