Product Pathways - Adhesion
EpCAM (D1B3) Rabbit mAb #2626
|2626S||100 µl (10 western blots)||---||In Stock||---|
|2626||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Monkey.
* Product-specific protocol.
Specificity / Sensitivity
EpCAM (D1B3) Rabbit mAb recognizes endogenous levels of total EpCAM protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human EpCAM protein.
Western blot analysis of extracts from MCF7 (EpCAM positive), HT-29 (EpCAM positive), and HeLa (EpCAM negative) cells using EpCAM (D1B3) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Flow cytometric analysis of HT-29 cells using EpCAM (D1B3) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).
Confocal immunofluorescent analysis of HT-29 (left) and HeLa (right) cells using EpCAM (D1B3) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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