Product Pathways - Apoptosis

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Atg5 Antibody #2630

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H Mk	Endogenous	55	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

2630:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Atg5 Antibody detects endogenous levels of total Atg5. The observed band represents the Atg12-Atg5 conjugated form, but the antibody likely reacts with free Atg5 as well.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of Atg5. Antibodies were purified by peptide affinity chromatography.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
4. Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92.
5. Mizushima, N. et al. (1998) Nature 395, 395-8.
6. Suzuki, K. et al. (2001) EMBO J 20, 5971-81.
7. Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79.
8. Shintani, T. et al. (1999) EMBO J 18, 5234-41.

Application References

• Zhou, W.J. et al. (2009) Mol Cancer Ther 8, 3203-13. Applications: Western Blotting
• Bommareddy, A. et al. (2009) Cancer Res 69, 3704-12. Applications: Western Blotting
• Yang, J. et al. (2010) J Cell Sci 123, 861-70. Applications: Western Blotting
• Lock, R. et al. (2011) Mol Biol Cell 22, 165-78. Applications: Western Blotting
• Han, W. et al. (2011) PLoS One 6, e18691. Applications: Western Blotting
• Wang, Z. et al. (2011) Autophagy 7, 401-11. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 2631 Atg7 Antibody
• 2010 Atg12 Antibody (Human Specific)
• 2011 Atg12 Antibody (Mouse Specific)
• 3738 Beclin-1 Antibody
• 2775 LC3B Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

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For Research Use Only. Not For Use In Diagnostic Procedures.