Product Pathways - Apoptosis / Autophagy
Atg7 Antibody #2631
PhosphoSitePlus® protein, site, and accession data: ATG7
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R (Mk) | Endogenous | 78 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2631:
- Western Blotting
Specificity / Sensitivity
Atg7 Antibody detects endogenous levels of total Atg7 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of Atg7. Antibodies were purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from THP-1 and BaF3 cells and rat spleen using Atg7 Antibody.
Background
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).
- Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
- Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
- Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
- Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92.
- Mizushima, N. et al. (1998) Nature 395, 395-8.
- Suzuki, K. et al. (2001) EMBO J 20, 5971-81.
- Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79.
- Shintani, T. et al. (1999) EMBO J 18, 5234-41.
Application References
- Zhang, Y. et al. (2009) Proc Natl Acad Sci U S A 106, 19860-5. Applications: Western Blotting
- Sahu, R. et al. (2011) Dev Cell 20, 131-9. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
