Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

BTAF1 Antibody #2637

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk (Hm) (B) (Hr) Endogenous 200 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  B=Bovine  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BTAF1 Antibody recognizes endogenous levels of total BTAF1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala220 of human BTAF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BTAF1 Antibody.

Background

B-TATA binding protein-associated factor 1 (BTAF1) is a human homolog of the yeast protein modifier of transcription 1 (MOT1) (1). BTAF1 negatively regulates transcription through interaction with TATA binding protein (TBP), an important component of the B-TFIID complex (2,3). BTAF1 is a member of the SNF2 family of ATPases that utilizes ATP to remove TBP from B-TFIID, thereby preventing formation of the transcription pre-initiation complex (PIC) (3-5). BTAF1 actively removes TBP from DNA, which prevents non-specific TBP-DNA interactions and promotes disassembly of inactive forms of the PIC. This activity is thought to be critical for maintaining a pool of free TBP that is necessary for the assembly of the PIC during transcription activation (6,7).

  1. Chicca, J.J. et al. (1998) Mol Cell Biol 18, 1701-10.
  2. Pereira, L.A. et al. (2004) J Biol Chem 279, 21802-7.
  3. Auble, D.T. (2009) Trends Biochem Sci 34, 49-52.
  4. Auble, D.T. et al. (1994) Genes Dev 8, 1920-34.
  5. de Graaf, P. et al. (2010) J Cell Sci 123, 2663-71.
  6. Dasgupta, A. et al. (2005) EMBO J 24, 1717-29.
  7. Huisinga, K.L. and Pugh, B.F. (2007) Genome Biol 8, R46.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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