Product Pathways - Tyrosine Kinase / Adaptors
VEGF Receptor 3 (C28G5) Rabbit mAb #2638
PhosphoSitePlus® protein, site, and accession data: VEGFR-3
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Transfected Only | 195 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2638:
- Western Blotting
Specificity / Sensitivity
VEGF Receptor 3 (C28G5) Rabbit mAb detects transfected levels of VEGFR3. This antibody does not cross-react with other family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly990 of human VEGF Receptor 3.
Western Blotting
Western blot analysis of extracts from COS cells transfected with human VEGFR3 expression constructs (right) and mock transfected COS cells (left) using VEGF Receptor 3 (C28G5) Rabbit mAb.
Background
Vascular endothelial growth factor receptor 3 (VEGFR3) is a 195 kDa membrane receptor tyrosine kinase. VEGF receptors are characterized by the presence of seven extracellular immunoglobulin (Ig)-like domains followed by a membrane-spanning domain and a conserved intracellular tyrosine kinase domain (1). VEGF receptor 3 expression is largely restricted to adult lymphatic endothelium and is thought to control lymphangiogenesis (1,2). Binding of VEGF-C/VEGF-D to VEGFR3 results in transphosphorylation of tyrosine residues in its intracellular domain, recruitment of signaling molecules and activation of ERK1/2 and Akt signaling cascades (1,3).
- Robinson, C.J. and Stringer, S.E. (2001) J. Cell Sci.114, 853-865.
- Valtola, R. et al. (1999) Am. J. Pathol. 154, 1381-1390.
- Saharinen, P. and Petrova, T.V. (2004) Ann. N.Y. Acad. Sci. 1014, 76-87.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
