Product Pathways - Cytoskeletal Signaling
Fibrillarin (C13C3) Rabbit mAb #2639
PhosphoSitePlus® protein, site, and accession data: FBL
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-IC | H M R Mk | Endogenous | 37 | Rabbit IgG |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Fibrillarin (C13C3) Rabbit mAb detects endogenous levels of total fibrillarin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Thr298 of human fibrillarin.
Western Blotting
Western blot analysis of extracts from various cell types using Fibrillarin (C13C3) Antibody.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Fibrillarin (C13C3) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Fibrillarin is a 2'-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place (1,2). Fibrillarin associates with several other structual proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2'-O-ribose methylation of targeted nucleotides within the rRNA sequence (3,4). The sequence, structure and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development (5).
- Tollervey, D. et al. (1993) Cell 72, 443-457.
- Wang, H. et al. (2000) EMBO J. 19, 317-323.
- Lafontaine, D.L. and Tollervey, D. (2000) Mol. Cell Biol. 20, 2650-2659.
- Galardi, S. et al. (2002) Mol. Cell Biol. 22, 6663-6668.
- Newton, K. et al. (2003) Mol. Cell Biol. 23, 8519-8527.
Application References
- Franklin, S. et al. (2011) Mol Cell Proteomics 10, M110.000703. Applications: Western Blotting
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
