Product Pathways - Neuroscience
α-Synuclein Antibody #2642
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PhosphoSitePlus® protein, site, and accession data: SNCA
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 18 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
α-Synuclein Antibody detects the alpha isoform of synuclein. The antibody may cross-react with β-synuclein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human α-synuclein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293, HeLa, rat brain and mouse brain cell extracts, using α-Synuclein Antibody.
IP
Immunoprecipitation of α-synuclein from HeLa cell extracts, using α-Synuclein Antibody. Lane 1 is lysate control, lane 2 is antibody alone and lane 3 is both antibody and lysate.
Background
α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-synuclein gene are linked to Parkinson's disease (1).
Application References
- Liang, T. et al. (2003) alpha-Synuclein maps to a quantitative trait locus for alcohol preference and is differentially expressed in alcohol-preferring and -nonpreferring rats. Proc. Natl. Acad. Sci. USA. 100, 4690-4695. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
