Product Pathways - Neuroscience
α-Synuclein Antibody #2642
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 18 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
α-Synuclein Antibody detects the alpha isoform of synuclein. The antibody may cross-react with β-synuclein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the carboxy-terminal sequence of human α-synuclein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293, HeLa, rat brain and mouse brain cell extracts, using α-Synuclein Antibody.
IP
Immunoprecipitation of α-synuclein from HeLa cell extracts, using α-Synuclein Antibody. Lane 1 is lysate control, lane 2 is antibody alone and lane 3 is both antibody and lysate.
Background
α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Mutations of the α-synuclein gene have been linked to Parkinson disease (1).
Application References
- Liang, T. et al. (2003) alpha-Synuclein maps to a quantitative trait locus for alcohol preference and is differentially expressed in alcohol-preferring and -nonpreferring rats. Proc. Natl. Acad. Sci. USA. 100, 4690-4695. This article references the use of α-Synuclein Antibody in the following applications: Western Blotting
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
