Cell Signaling Technology

Product Pathways - Neuroscience

α/β-Synuclein (Syn205) Mouse mAb #2644

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-F H M R Endogenous 18 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

α/β-Synuclein (Syn205) Mouse mAb detects endogenous levels of total synuclein protein. α/β-Synuclein (Syn205) Mouse mAb detects recombinant α and β-synuclein (Giasson, B.I. et al., 2000).

Source / Purification

Monoclonal antibody is produced by immunizing animals with human recombinant α- and β-synucleins and the epitope maps to the carboxy terminus of the protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain, using α/β-Synuclein (Syn205) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain, using α/β-Synuclein (Syn205) Mouse mAb.

IF-F

IF-F

Confocal immunofluorescence images of rat hippocampus labeled with α/β-Synuclein (Syn205) Mouse mAb (red) and CREB (48H2) Rabbit mAb #9197 (green).


Background

α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-synuclein gene are linked to Parkinson's disease (1).

  1. Goldberg, M.S. and Lansbury Jr., P.T. (2000) Nat. Cell Biol. 2, 115-119.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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