Cell Signaling Technology

Product Pathways - DNA Damage

Trf2 Antibody #2645

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk Endogenous 65 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Trf2 Antibody detects endogenous levels of Trf2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide correspoding to residues near the carboxy terminus of human Trf2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7, HT29 and RD-ES cells, using Trf2 Antibody.

IP

IP

Western blot analysis of whole cell lysate (lane 1) and Trf2 immunoprecipitated from RD-ES cells (lane 2), using Trf2 Antibody.

Background

Telomeres, the linear ends of chromosomes, are organized into T-loops to prevent them from being recognized by the cell as DNA double stranded breaks (DSBs) (1). The telomeric repeat binding factor proteins, Trf1 and Trf2, bind to double-stranded telomeres, allowing formation of T-loops (2). A large number of proteins involved in the DNA damage response are found present within telomeres physically associated with Trf2 (3). Interestingly, Trf2 can be observed to transiently localize to DNA damage-induced DSBs, but overexpression of Trf2 prevents ATM-dependent signaling (4). Phosphorylation of Trf2 at Ser323 has been reported in vivo, but no upstream kinase or role has been established for this phosphorylation site (5).

  1. Karlseder, J. et al. (1999) Science 283, 1321-1325.
  2. Yoshimura, S. H. et al. (2004) Genes Cells 9, 205-218.
  3. Colgin, L. and Reddel, R. (2004) Curr. Biol. 14, R901-R902.
  4. Bradshaw, P. S. et al. (2005) Nat. Genet. 37, 193-197.
  5. Beausoleil, S. A. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 12130-12135.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products