Cell Signaling Technology

Product Pathways - Neuroscience

α-Synuclein (Syn204) Mouse mAb #2647

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P H Endogenous 18 Mouse IgG2a

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

α-Synuclein (Syn204) Mouse mAb detects endogenous levels of total synuclein protein. α-Synuclein (Syn204) Mouse mAb detects recombinant α but not β-synuclein (Giasson, B.I. et al., 2000).

Source / Purification

Monoclonal antibody is produced by immunizing animals with human recombinant α-synuclein and the epitope maps to the amino-terminus of the protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from human cerebellum and HeLa cells, using α-Synuclein (Syn204) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human brain, using α-Synuclein (Syn204) Mouse mAb.

IF-P

IF-P

Confocal immunofluorescent image of human Alzheimer's brain labeled with α-Synuclein (Syn204) Mouse mAb (red) and β-Amyloid Antibody #2454 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-synuclein gene are linked to Parkinson's disease (1).

  1. Goldberg, M.S. and Lansbury Jr., P.T. (2000) Nat. Cell Biol. 2, 115-119.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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