Cell Signaling Technology

Product Pathways - Protein Stability

VCP Antibody #2648

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC F H M R Mk (X) (Z) (B) (Pg) (Sc) Endogenous 89 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  X=Xenopus  Z=Zebrafish  B=Bovine  Pg=Pig  Sc=S. cerevisiae
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

This antibody detects endogenous levels of total VCP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human VCP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, K562, NIH/3T3, C6 and COS cells, using VCP Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K562 cells, using VCP Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Immunofluorescence staining of paraformaldehyde-fixed HeLa cells, using VCP Antibody.


Background

Valosin-containing protein (VCP) is a highly conserved and abundant 97 kDa protein that belongs to the AAA (ATPase associated with a variety of cellular activities) family of proteins. VCP assembles as a homo-hexamer, forming a ring with a channel at its center (1,2,3). VCP homo-hexamers associate with a variety of protein cofactors to form many distinct protein complexes, which act as chaperones to unfold proteins and transport them to specific cellular compartments or to the proteosome (4). These protein complexes participate in many cellular functions, including vesicle transport and fusion, fragmentation and reassembly of the golgi stacks during mitosis, nuclear envelope formation and spindle disassembly following mitosis, cell cycle regulation, DNA damage repair, apoptosis, B- and T-cell activation, NF-κB-mediated transcriptional regulation, endoplasmic reticulum (ER)-associated degradation and protein degradation (4). VCP appears to localize mainly to the endoplasmic reticulum; however, tyrosine phosphorylation is associated with relocalization to the centrosome during mitosis (5). In addition, following cellular exposure to ionizing radition, VCP is phosphorylated at Ser784 in an ATM-dependent manner and accumulates in the nucleus at sites of double-stranded DNA breaks (DSBs) (6). Exposure to other types of DNA damaging agents such as UV light, bleomycin or doxorubicin results in phosphorylation of VCP by ATR and DNA-PK in an ATM-independent manner (6).

  1. DeLaBarre, B. and Brunger, A.T. (2003) Nat. Struct. Biol. 10, 856-863.
  2. Huyton, T. et al. (2003) J. Struct. Biol. 144, 337-348.
  3. Dreveny, I. et al. (2004) EMBO J. 23, 1030-1039.
  4. Wang, Q. et al. (2004) J. Struct. Biol. 146, 44-57.
  5. Madeo, F. et al. (1998) Mol. Biol. Cell 9, 131-141.
  6. Livingstone, M. et al. (2005) Cancer Res. 65, 7533-7540.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products