Product Pathways - Protein Stability
VCP (7F3) Rabbit mAb #2649
|2649S||100 µl (10 western blots)||---||In Stock||---|
|2649||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||89||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Xenopus, Zebrafish, Bovine, Pig, S. cerevisiae.
Specificity / Sensitivity
This antibody detects endogenous levels of total VCP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human VCP.
Valosin-containing protein (VCP) is a highly conserved and abundant 97 kDa protein that belongs to the AAA (ATPase associated with a variety of cellular activities) family of proteins. VCP assembles as a homo-hexamer, forming a ring with a channel at its center (1,2,3). VCP homo-hexamers associate with a variety of protein cofactors to form many distinct protein complexes, which act as chaperones to unfold proteins and transport them to specific cellular compartments or to the proteosome (4). These protein complexes participate in many cellular functions, including vesicle transport and fusion, fragmentation and reassembly of the golgi stacks during mitosis, nuclear envelope formation and spindle disassembly following mitosis, cell cycle regulation, DNA damage repair, apoptosis, B- and T-cell activation, NF-κB-mediated transcriptional regulation, endoplasmic reticulum (ER)-associated degradation and protein degradation (4). VCP appears to localize mainly to the endoplasmic reticulum; however, tyrosine phosphorylation is associated with relocalization to the centrosome during mitosis (5). In addition, following cellular exposure to ionizing radition, VCP is phosphorylated at Ser784 in an ATM-dependent manner and accumulates in the nucleus at sites of double-stranded DNA breaks (DSBs) (6). Exposure to other types of DNA damaging agents such as UV light, bleomycin or doxorubicin results in phosphorylation of VCP by ATR and DNA-PK in an ATM-independent manner (6).
- DeLaBarre, B. and Brunger, A.T. (2003) Nat. Struct. Biol. 10, 856-863.
- Huyton, T. et al. (2003) J. Struct. Biol. 144, 337-348.
- Dreveny, I. et al. (2004) EMBO J. 23, 1030-1039.
- Wang, Q. et al. (2004) J. Struct. Biol. 146, 44-57.
- Madeo, F. et al. (1998) Mol. Biol. Cell 9, 131-141.
- Livingstone, M. et al. (2005) Cancer Res. 65, 7533-7540.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2648 VCP Antibody
- 4526 Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb
- 2853 Phospho-ATR (Ser428) Antibody
- 4602 DNA-PK Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.