Cell Signaling Technology

Product Pathways - Protein Stability

USP4 Antibody #2651

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk (Dg) Endogenous 104, 109 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

USP4 Antibody recognizes endogenous levels of total USP4 protein. Based upon sequence alignment, this antibody is predicted to cross-react with the UnpEL and UnpES isoforms of USP4. This antibody does not cross-react with USP15.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His666 of human USP4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using USP4 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human USP4, isoform1/UnpEL (hUSP4-Myc/DDK; +), using USP4 Antibody.

Background

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. USP4 was originally identified during a survey of murine genes near the Mpv20 retroviral insertion site and intially referred to as Ubiquitous Nuclear Protein (UNP). Analysis of the mouse cDNA originally identified Usp4/Unp as a proto-oncogene related to the human tre-2/tre-17/USP6 proto-oncogene (3,4). Usp4/Unp was subsequently observed to contain the conserved Cys and His boxes of the UBP family (5,6) as well as DUB activity (7,8). In a study of primary lung tumor tissue, it was observed that the human homolog of Usp4, USP4/UNPH, had elevated gene expression levels in small cell tumors and adenocarcinomas of the lung, suggesting a causative role for USP4 in neoplasia (6). Another recent study demonstrated overexpression of USP4 in several types of human cancer and that USP4 positively contributes to cell transformation by negatively regulating p53 levels (9). Both murine and human USP4 have been shown to interact with the Rb family of tumor suppressor proteins, providing additional mechanistic evidence of a role for USP4 in cellular transformation (10, 11).

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Gupta, K. et al. (1994) Oncogene 9, 1729-31.
  4. Gupta, K. et al. (1993) Oncogene 8, 2307-10.
  5. Papa, F.R. and Hochstrasser, M. (1993) Nature 366, 313-9.
  6. Gray, D.A. et al. (1995) Oncogene 10, 2179-83.
  7. Gilchrist, C.A. et al. (1997) J Biol Chem 272, 32280-5.
  8. Gilchrist, C.A. and Baker, R.T. (2000) Biochim Biophys Acta 1481, 297-309.
  9. Zhang, X. et al. (2011) EMBO J 30, 2177-89.
  10. Blanchette, P. et al. (2001) Oncogene 20, 5533-7.
  11. DeSalle, L.M. et al. (2001) Oncogene 20, 5538-42.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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