Product Pathways - Development
CK1 Antibody #2655
PhosphoSitePlus® protein, site, and accession data: CK1-A
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R (C) (X) (Z) (B) | Endogenous | 34 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
C=Chicken
X=Xenopus
Z=Zebrafish
B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2655:
- Western Blotting
Specificity / Sensitivity
This antibody detects endogenous levels of total casein kinase I-alpha 1 protein. This antibody may cross-react with casein kinase I-epsilon, casein kinase I-delta, and casein kinase I-gamma.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid residues near the middle of human casein kinase I-alpha 1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of lysates from various cell lines using Casein Kinase I Antibody.
Background
Casein Kinase I (CK1 or CKI) is the name given to a family of kinases consisting of multiple isoforms (α, α', β, γ1-3, δ, and ε) with a conserved N-terminal kinase domain and a variable C-terminal sequence that determines subcellular localization and regulates enzyme activity (1-3). Indeed, multiple inhibitory autophosphorylation sites have been identified near the C terminus of CK1ε (3). This ubiquitously expressed family of protein kinases has been implicated in multiple processes including DNA repair, cell morphology, and Wnt signaling (4). Perhaps the best understood role of CK1 is to provide the priming phosphorylation of β-catenin at Ser45 to produce the consensus GSK-3 substrate motif (S/T-X-X-X-pS) (4).
- Gross, S.D. and Anderson, R.A. (1998) Cell. Signal. 10, 699-711.
- Vancura, A. et al. (1994) J. Biol. Chem. 269, 19271-19278.
- Gietzen, K.F. and Virshup, D.M. (1999) J. Biol. Chem. 274, 32063-32070.
- Polakis, P. (2002) Curr. Biol. 12, R499-R501.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
