Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

CK1 Antibody #2655

Applications Reactivity MW (kDa) Source
W H M R (C) (B) (X) 34 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  C=Chicken  B=Bovine  X=Xenopus
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

This antibody detects endogenous levels of total casein kinase I-alpha 1 protein. This antibody may cross-react with casein kinase I-epsilon, casein kinase I-delta, and casein kinase I-gamma.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acid residues near the middle of human casein kinase I-alpha 1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of lysates from various cell lines using Casein Kinase I Antibody.

Background

CK1 (Casein Kinase I or CKI) is the name given to a family of kinases consisting of multiple isoforms (alpha, alpha-prime, beta, gamma1-3, delta, and epsilon) with a conserved N-terminal kinase domain and a variable C-terminal sequence that determines subcellular localization and regulates enzyme activity (1-3). Indeed, multiple inhibitory autophosphorylation sites have been identified near the C-terminus of (CKI-epsilon) (3). This ubiquitously expressed family of protein kinases has been implicated in multiple processes including DNA repair, cell morphology, and Wnt signaling (4). Perhaps the best understood role of CKI is to provide the priming phosphorylation of beta-catenin at Ser45 to produce the consensus GSK-3 substrate motif (Ser/Thr-X-X-X-Phospho-Ser) (4).

  1. Gross, S.D. and Anderson, R.A. (1998) Cell. Signal. 10, 699-711.
  2. Vancura, A. et al. (1994) J. Biol. Chem. 269, 19271-19278.
  3. Gietzen, K.F. and Virshup, D.M. (1999) J. Biol. Chem. 274, 32063-32070.
  4. Polakis, P. (2002) Curr. Biol. 12, R499-R501.

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