Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CK2α Antibody #2656

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 42 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

This antibody detects endogenous levels of total CK2α 1 protein. This antibody may cross-react with CK2α prime.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid residues near the carboxy-terminus of human CK2α1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of lysates from various cell lines using CK2α Antibody.

Background

CK2 (formerly called Casein Kinase II) is a highly conserved protein kinase with more than 300 substrates regulating cell growth, cell death, and cell survival. CK2 has been implicated in the response to UV irradiation-induced DNA damage, targeting XRCC1 (1) and BRCA1 (2) as well as regulating p53 tumor suppressor protein functions (3). Furthermore, CK2 plays a key role in NF-κB activation (4). UV irradiation stimulates CK2-mediated phosphorylation of several carboxy-terminal residues within IκBα, resulting in IκBα proteasomal degradation and the release and nuclear translocation of active NF-κB. CK2 is also dysregulated in many cancers (5) and neurodegenerative diseases such as Alzheimer's and Parkinson's diseases (6). Structurally, CK2 is a multimeric protein complex consisting of two catalytic subunits (α or α') and two regulatory β subunits (7). CK2 is distributed ubiquitously and is apparently constitutively active (7). While cell cycle-dependent Ser-Pro phosphorylation sites have been identified on CK2α and CK2β, Tyr255 phosphorylation by the Src-related kinase c-Fgr seems to have the greatest effect on CK2α activity (8,9).

  1. Morales, J.C. and Carpenter, P.B. (2004) Sci Aging Knowledge Environ 2004, pe24.
  2. O'Brien, K.A. et al. (1999) Biochem Biophys Res Commun 260, 658-64.
  3. Cox, M.L. and Meek, D.W. (2010) Cell Signal 22, 564-71.
  4. Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7.
  5. Trembley, J.H. et al. Biofactors 36, 187-95.
  6. Perez, D.I. et al. (2010) Med Res Rev, Epub ahead of print.
  7. Bosc, D.G. et al. (1995) J Biol Chem 270, 25872-8.
  8. Donella-Deana, A. et al. (2003) Biochem J 372, 841-9.
  9. Litchfield, D.W. (2003) Biochem J 369, 1-15.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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