Product Pathways - Cell Cycle / Checkpoint
CK2α Antibody #2656
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H M R Mk | 42 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
This antibody detects endogenous levels of total CK2α 1 protein. This antibody may cross-react with CK2α prime.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acid residues near the carboxy-terminus of human CK2α1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of lysates from various cell lines using CK2α Antibody.
Background
CK2 (formerly called Casein Kinase II) is a multimeric protein complex consisting of two catalytic subunits (α or α') and two regulatory β subunits (1). CK2 is distributed ubiquitously and apparently constitutively active (1). While cell cycle-dependent Ser-Pro phosphorylation sites have been identified on CK2α and CK2beta, Tyr255 phosphorylation by the Src-related kinase c-Fgr seems to most regulate CK2α activity (2,3).
- Litchfield, D. W. et al. (1995) J. Biol. Chem. 270, 25872-25878.
- Donella-Deana, A. et al. (2003) Biochem. J. 372, 841-849.
- Litchfield, D.W. (2003) Biochem. J. 369, 1-15.
Application References
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