Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Lck (L22B1) Mouse mAb #2657

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 56 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Lck (L22B1) Mouse mAb detects endogenous levels of total Lck protein. This antibody does not cross-react with other Src family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-Lck fusion protein containing amino acid residues 1 to 234 of human Lck.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Lck (L22B1) Mouse mAb.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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