Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Lck (L22B1) Mouse mAb #2657

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 56 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Lck (L22B1) Mouse mAb detects endogenous levels of total Lck protein. This antibody does not cross-react with other Src family members.

Source / Purification

Monoclonal antibodies are produced by immunizing mice with a GST-Lck fusion protein containing amino acid residues 1 to 234 of human Lck.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Lck (L22B1) Mouse mAb.

Background

The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk and Hck) are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain by Csk upregulates enzyme activity, whereas phosphorylation of Tyr527 in the carboxy-terminal tail renders the enzyme less active (2).

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

Products