Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

Deltex-2 Antibody #2663

Applications Reactivity Sensitivity MW (kDa) Source
W IP M (H) (R) Transfected Only 70 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Deltex-2 Antibody detects transfected levels of total Deltex-2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala607 of human Deltex-2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transfected with mouse Deltex-2, using Deltex-2 Antibody.

Background

Deltex (DTX) encodes a putative E3 ubiquitin ligase first identified from Drosophila and shown to modulate Notch signaling (1). Several mammalian Deltex homologs have been isolated (2,3), all containing a pair of WWE repeats, a proline-rich region, and a Ring-finger domain (4,5). Deltex binds to the ankyrin-repeat domain of Notch and is capable of regulating Notch signaling positively or negatively in different systems (4,6). In addition, Deltex can also form homo-multimers (4). While Deltex has been implicated in Notch signaling regulation of lymphocyte development, this signal modifier may also have a transcriptional function independent of Notch (3, 6-8).

  1. Xu, T. and Artavanis-Tsakonas, S. (1990) Genetics 126, 665-77.
  2. Kishi, N. et al. (2001) Int J Dev Neurosci 19, 21-35.
  3. Lehar, S.M. and Bevan, M.J. (2006) Mol Cell Biol 26, 7358-71.
  4. Matsuno, K. et al. (1995) Development 121, 2633-44.
  5. Zweifel, M.E. et al. (2005) Structure 13, 1599-611.
  6. Izon, D.J. et al. (2002) Immunity 16, 231-43.
  7. Yun, T.J. and Bevan, M.J. (2003) J Immunol 170, 5834-41.
  8. Yamamoto, N. et al. (2001) J Biol Chem 276, 45031-40.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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