Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677

Applications Reactivity MW (kDa) Source Isotype
IP IF-IC F H (M) (R) (Mk) (Pg) 92 Mouse IgG1

Applications Key:  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Pg=Pig
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

β-Catenin (L54E2) Mouse mAb (IF Preferred) detects endogenous levels of total β-catenin protein.

Source / Purification

Monoclonal antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of human β-catenin.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NCI-H28 cells (blue) and HeLa cells (green) using β-Catenin (L54E2) Mouse mAb (IF Preferred).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa (left) and NCI-H28 cells (right) using β-Catenin (L54E2) Mouse mAb(IF Preferred) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin on Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37 and Thr41 (7). Mutations in these phosphorylation sites, which result in the stabilization of β-catenin protein levels, have been found in many tumor cell lines (8).

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
  2. Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
  3. Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
  5. Lin, C. et al. (2002) Cell 108, 837-847.
  6. Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
  7. Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
  8. Morin, P.J. (1997) Science 275, 1787-1790.

Application References

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