Cell Signaling Technology

Product Pathways - NF-kappaB Signaling

IKKβ (L570) Antibody (IP Preferred) #2678

Applications Reactivity MW (kDa) Source
W IP H M R Mk Hm B 87 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Hm=Hamster  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

IKKβ (L570) (IP Preferred) Antibody detects endogenous levels of total IKKβ protein.

Source / Purification

Polyclonal antibodies are prepared by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding leucine 570 of IKKβ. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 and A20 cell lines, using IKKβ (L570) (IP Preferred) Antibody.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase. IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends on phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13).

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