Product Pathways - NF-kB Signaling

• pathway
• application references
• companion products
• datasheet PDF
• MSDS PDF
• protocols

Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IHC-P IHC-F	H M R Mk (B)	Endogenous	85 IKK-alpha 87 IKK-beta	Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

2697:

IHC / Frozen, IHC / Paraffin, Western Blotting

Specificity / Sensitivity

Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb detects IKKα only when phosphorylated at Ser176/180 and IKKβ only when phosphorylated at Ser177/181.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser176/180 of human IKKα.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Zandi, E. et al. (1997) Cell 91, 243-52.
9. Karin, M. (1999) Oncogene 18, 6867-74.
10. DiDonato, J.A. et al. (1997) Nature 388, 548-54.
11. Mercurio, F. et al. (1997) Science 278, 860-6.
12. Johnson, L.N. et al. (1996) Cell 85, 149-58.
13. Delhase, M. et al. (1999) Science 284, 309-13.

Application References

• Kwong, C. et al. (2011) J Immunol 186, 1781-9. Applications: Western Blotting
• Barisic, S. et al. (2010) Biochem Pharmacol 80, 439-47. Applications: Western Blotting
• Iwasaki, H. et al. (2011) Nat Immunol 12, 1167-75. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 2370 IKKβ (2C8) Rabbit mAb
• 2682 IKKα Antibody
• 2078 Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb
• 2694 Phospho-IKKα/β (Ser176/180) Antibody II
• 2684 IKKβ Antibody
• 2678 IKKβ (L570) Antibody
• 1023 Phospho-IKK-alpha/beta (Ser176/180) Blocking Peptide
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
• 5425 Normal Goat Serum

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

---

For Research Use Only. Not For Use In Diagnostic Procedures.