Product Pathways - NF-kappaB Signaling

Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697

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Applications	Reactivity	MW (kDa)	Source	Isotype
W IHC-P IHC-F	H M R Mk (B)	85 IKK-alpha 87 IKK-beta	Rabbit	IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb detects IKKα only when phosphorylated at Ser176/180 and IKKβ only when phosphorylated at Ser177/181.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser176/180 of human IKKα.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase. IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends on phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13).

1. Baeuerle, P.A. et al. (1988) Science 242, 540-546.
2. Beg, A.A. et al. (1993) Genes Dev. 7, 2064-2070.
3. Finco, T.S. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 11884-11888.
4. Brown, K. et al. (1995) Science 267, 1485-1488.
5. Brockman, J.A. et al. (1995) Mol. Cell. Biol. 15, 2809-2818.
6. Traenckner, E.B. et al. (1995) EMBO J. 14, 2876-2883.
7. Chen, Z.J. et al. (1996) Cell 84, 853-862.
8. Zandi, E. et al. (1997) Cell 91, 243-252.
9. Karin, M. et al. (1999) Oncogene 18, 6867-6874.
10. DiDonato, J.A. et al. (1997) Nature 388, 548-554.
11. Mercurio, F. et al. (1997) Science 278, 860-866.
12. Johnson, L.N. et al. (1996) Cell 85, 149-158.
13. Delhase, M. et al. (1999) Science 284, 309-313.

Application References

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Companion Products

• 2687 Phospho-IKKα/β (Ser176/180) Antibody
• 2694 Phospho-IKKα/β (Ser176/180) Antibody II
• 2681 Phospho-IKKα (Ser180)/IKKβ (Ser181) Antibody
• 2682 IKKα Antibody
• 2684 IKKβ Antibody
• 2685 IKKγ Antibody
• 1023 Phospho-IKK-alpha/beta (Ser176/180) Blocking Peptide
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.