Product Pathways - Lymphocyte Signaling
Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody #2701
|2701L||300 µl (30 western blots)||---||In Stock||---|
|2701S||100 µl (10 western blots)||---||In Stock||---|
|2701P||40 µl (4 western blots)||---||In Stock||---|
|2701||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human||Endogenous||70 Zap-70, 72 Syk||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Mouse, Rat, Hamster, Monkey, Chicken, Bovine, Dog, Pig, Horse.
Specificity / Sensitivity
Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. It cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from Jurkat cells, starved for 16 hours, and treated with 2 mM H2O2 or with calf intestinal alkaline phosphatase (CIP), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody (upper) or control Zap-70 Antibody #2702 (lower).
Western blot analysis of extracts from Ramos cells, untreated or treated with anti-IgM (12 µg/ ml for 2 minutes), hydrogen peroxide (10 mM for 2 minutes) or lambda phosphatase, using Phospho-Zap-70 (Tyr319)/ Syk (Tyr352) Antibody.
Two-color flow cytometric analysis of Jurkat cells, untreated (left) or anti-CD3 activated (right), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody and Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) mAb #9106. Anti-CD3 activation increases the intensity of label with both antibodies.
Flow cytometric analysis of Jurkat cells, untreated (blue) or CD3 treated (green), using Phospho-Zap70 (Tyr319)/Syk (Tyr352) Antibody compared to a nonspecific negative control antibody (red).
The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
Phosphorylation of Tyr319 is required for the assembly of a Zap-70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells (5,6). The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1 (9).
- Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
- Iwashima, M. et al. (1994) Science 263, 1136-1139.
- Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
- Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
- Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
- Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
- Wiestner, A. et al. (2003) Blood 101, 4944-4951.
- Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.
- Law, C. L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.
- Guntermann, C. and Alexander, D.R. (2002) J. Immunol. 168, 4420-4429. Applications: Western Blotting, IP.
- Houtman, J. C. et al. (2005) J Immunol. 175 (4), 2449-2458. Applications: Western Blotting.
- Schade, A.E. and Levine, A.D. (2002) . J. Immunol. 168, 2233-2239. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.