Product Pathways - Lymphocyte Signaling
Zap-70 (99F2) Rabbit mAb #2705
|2705S||100 µl (10 western blots)||---||In Stock||---|
|2705||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||70||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry
Specificity / Sensitivity
Zap-70 (99F2) Rabbit mAb detects endogenous levels of total Zap-70.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino-terminus of human Zap-70.
Western blot analysis of extracts from Jurkat cells, starved for 16 hours and either untreated or H2O2-treated (2 mM for 3 minutes), using Zap-70 (99F2) Rabbit mAb.
Immunofluorescent staining of paraffin-embedded human tonsil, showing Zap-70 localized with T-cells surrounding a germinal center, using Zap-70 (99F2) Rabbit mAb (green) and anti-CD3 (T-cell marker) (red). DAPI stain is shown in blue.
Immunohistochemical analysis of paraffin-embedded Jurkat T cells (left) and Ramos B cells (right), using Zap-70 (99F2) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human tonsil, using Zap-70 (99F2) Rabbit mAb.
The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
- Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
- Iwashima, M. et al. (1994) Science 263, 1136-1139.
- Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
- Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
- Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
- Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
- Wiestner, A. et al. (2003) Blood 101, 4944-4951.
- Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.