Product Pathways - Lymphocyte Signaling
Zap-70 (99F2) Rabbit mAb #2705
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W IP IHC-P F | H M | Endogenous | 70 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Zap-70 (99F2) Rabbit mAb detects endogenous levels of total Zap-70.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding the amino-terminus of human Zap-70.
Western Blotting
Western blot analysis of extracts from Jurkat cells, starved for 16 hours and either untreated or H2O2-treated (2 mM for 3 minutes), using Zap-70 (99F2) Rabbit mAb.
IHC-P (paraffin)
Immunofluorescent staining of paraffin-embedded human tonsil, showing Zap-70 localized with T-cells surrounding a germinal center, using Zap-70 (99F2) Rabbit mAb (green) and anti-CD3 (T-cell marker) (red). DAPI stain is shown in blue.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Jurkat T cells (left) and Ramos B cells (right), using Zap-70 (99F2) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human tonsil, using Zap-70 (99F2) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Ramos B cells (blue) and Jurkat T cells (green), using Zap-70 (99F2) Rabbit mAb.
Background
Zap-70, a Syk family protein tyrosine kinase expressed in T and NK cells, plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues, presumably by two mechanisms: autophosphorylation and transphosphorylation by the Src family tyrosine kinase, Lck (2-6). Tyrosine phosphorylation of Zap-70 correlates with its increased kinase activity and downstream signaling events. In patients with chronic lymphocytic leukemia (CLL), total Zap-70 expression was shown to be correlated with disease progression and survival (7,8).
- Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
- Iwashima, M. et al. (1994) Science 263, 1136-1139.
- Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
- Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
- Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
- Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
- Wiestner, A. et al. (2003) Blood 101, 4944-4951.
- Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.
Application References
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Companion Products
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- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2707 Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 8112 SignalStain® Antibody Diluent
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
