Product Pathways - Lymphocyte Signaling
Zap-70 (99F2) Rabbit mAb #2705
PhosphoSitePlus® protein, site, and accession data: ZAP70
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P F | H M | Endogenous | 70 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Zap-70 (99F2) Rabbit mAb detects endogenous levels of total Zap-70.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino-terminus of human Zap-70.
Western Blotting
Western blot analysis of extracts from Jurkat cells, starved for 16 hours and either untreated or H2O2-treated (2 mM for 3 minutes), using Zap-70 (99F2) Rabbit mAb.
IHC-P (paraffin)
Immunofluorescent staining of paraffin-embedded human tonsil, showing Zap-70 localized with T-cells surrounding a germinal center, using Zap-70 (99F2) Rabbit mAb (green) and anti-CD3 (T-cell marker) (red). DAPI stain is shown in blue.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Jurkat T cells (left) and Ramos B cells (right), using Zap-70 (99F2) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human tonsil, using Zap-70 (99F2) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Ramos B cells (blue) and Jurkat T cells (green), using Zap-70 (99F2) Rabbit mAb.
Background
The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
- Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
- Iwashima, M. et al. (1994) Science 263, 1136-1139.
- Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
- Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
- Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
- Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
- Wiestner, A. et al. (2003) Blood 101, 4944-4951.
- Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.
Application References
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Companion Products
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- 8112 SignalStain® Antibody Diluent
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- 5425 Normal Goat Serum
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.
