Product Pathways - Lymphocyte Signaling
Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) #2707
PhosphoSitePlus® protein, site, and accession data: ZAP70
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| F | H M | Endogenous | 70 | Rabbit IgG |
Applications Key:
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2707:
- Flow
Specificity / Sensitivity
Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total Zap-70 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino-terminus of human Zap-70. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.
Flow Cytometry
Flow cytometric analysis of Ramos B (blue) and Jurkat T (green) cells using Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate).
Flow Cytometry
Two-color flow cytometric analysis of a mixed population of T and B cells (Jurkat and Ramos, respectively) using Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) and a CD3 antibody. CD3 negative B cells have little or no Zap-70 staining, while CD3 positive T cells stain brightly for Zap-70 protein.
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells.
Background
The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
- Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
- Iwashima, M. et al. (1994) Science 263, 1136-1139.
- Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
- Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
- Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
- Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
- Wiestner, A. et al. (2003) Blood 101, 4944-4951.
- Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2701 Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody
- 2704 Phospho-Zap-70 (Tyr493)/Syk (Tyr526) Antibody
- 2705 Zap-70 (99F2) Rabbit mAb
- 2985 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate)
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.