Product Pathways - Lymphocyte Signaling
Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) #2707
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| F | H | Endogenous | 70 | Rabbit | IgG |
Applications Key:
F=Flow Cytometry
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Zap-70 (136F12) Rabbit mAb (Alexa Fluor®647 Conjugate) detects endogenous levels of total Zap-70
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to residues surrounding the amino-terminus of human Zap-70. The antibody was conjugated to Alexa Fluor®647 under optimum conditions with an F/P ratio of 2-6.
Flow Cytometry
Flow cytometric analysis of Ramos B cells (blue) and Jurkat T cells (green), using Zap-70(136F12) Rabbit mAb (Alexa Fluor®647) Conjugate.
Flow Cytometry
Two-color flow cytometric analysis of a mixed population of T and B cells (Jurkat and Ramos, respectively), using Zap-70(136F12) Rabbit mAb (Alexa Fluor®647) Conjugate and a CD3 antibody. CD3 negative B cells have little or no Zap-70 staining, while CD3 positive T cells stain brightly for Zap-70.
Description
Cell Signaling Technology Antibody conjugated to Alexa Fluor®647 fluorescent dye and tested in-house for direct Flow cytometric analysis of human cells.
Background
Zap-70, a Syk family protein tyrosine kinase expressed in T and NK cells, plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues, presumably by two mechanisms: autophosphorylation and transphosphorylation by the Src family tyrosine kinase, Lck (2-6). Tyrosine phosphorylation of Zap-70 correlates with its increased kinase activity and downstream signaling events. In patients with chronic lymphocytic leukemia (CLL), total Zap-70 expression was shown to be correlated with disease progression and survival (7,8).
- Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
- Iwashima, M. et al. (1994) Science 263, 1136-1139.
- Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
- Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
- Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
- Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
- Wiestner, A. et al. (2003) Blood 101, 4944-4951.
- Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2705 Zap-70 (99F2) Rabbit mAb
- 2701 Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody
- 2704 Phospho-Zap-70 (Tyr493) Antibody
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
