Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) #2707

Applications Reactivity Sensitivity MW (kDa) Source Isotype
F H Endogenous 70 Rabbit IgG

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Zap-70 (136F12) Rabbit mAb (Alexa Fluor®647 Conjugate) detects endogenous levels of total Zap-70

Source / Purification

Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to residues surrounding the amino-terminus of human Zap-70. The antibody was conjugated to Alexa Fluor®647 under optimum conditions with an F/P ratio of 2-6.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ramos B cells (blue) and Jurkat T cells (green), using Zap-70(136F12) Rabbit mAb (Alexa Fluor®647) Conjugate.

Flow Cytometry

Flow Cytometry

Two-color flow cytometric analysis of a mixed population of T and B cells (Jurkat and Ramos, respectively), using Zap-70(136F12) Rabbit mAb (Alexa Fluor®647) Conjugate and a CD3 antibody. CD3 negative B cells have little or no Zap-70 staining, while CD3 positive T cells stain brightly for Zap-70.

Description

Cell Signaling Technology Antibody conjugated to Alexa Fluor®647 fluorescent dye and tested in-house for direct Flow cytometric analysis of human cells.

Background

Zap-70, a Syk family protein tyrosine kinase expressed in T and NK cells, plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues, presumably by two mechanisms: autophosphorylation and transphosphorylation by the Src family tyrosine kinase, Lck (2-6). Tyrosine phosphorylation of Zap-70 correlates with its increased kinase activity and downstream signaling events. In patients with chronic lymphocytic leukemia (CLL), total Zap-70 expression was shown to be correlated with disease progression and survival (7,8).

  1. Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
  2. Iwashima, M. et al. (1994) Science 263, 1136-1139.
  3. Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
  4. Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
  5. Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
  6. Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
  7. Wiestner, A. et al. (2003) Blood 101, 4944-4951.
  8. Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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