Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb #2710

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H (M) (R) Endogenous 72 kDa Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb detects endogenous levels of Syk protein only when phosphorylated at Tyr525/526 of human Syk or Tyr519/520 of mouse Syk. It also detects Syk protein when singly phosphorylated at Tyr526 of human Syk or Tyr520 of mouse Syk. It does not cross-react with other tyrosine-phosphorylated protein tyrosine kinases.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr525/526 of human Syk.

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos cells, untreated or treated with anti-IgM, using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (upper) or Syk Antibody #2712 (lower).

Background

Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation of Tyr323 provides a direct binding site to the TKB domain of Cbl (6,7). Tyrosine 352 of Syk is involved in the association of PLC-γ1 (8). Tyrosines 525 and 526 are located in the activation loop of the Syk kinase domain, and phosphorylation of Tyr525/526 of human Syk (equivalent to the Tyr519/520 of mouse Syk) is essential for Syk function (9).

  1. Cheng, A.M. and Chan, A.C. (1997) Curr. Opin. Immunol. 9, 528-533.
  2. Kurosaki, T. et al. (1997) Curr. Opin. Immunol. 9, 309-318.
  3. Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
  4. Turner, M. et al. (2000) Immunol. Today 21, 148-154.
  5. Coopman, P.J. et al. (2000) Nature 406, 742-747.
  6. Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.
  7. Rao, N. et al. (2001) EMBO J. 20, 7085-7095.
  8. Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.
  9. Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

Products