Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-Syk (Tyr525/526) Antibody #2711

Applications Reactivity Sensitivity MW (kDa) Source
W H (M) (R) Endogenous 72 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Syk (Tyr525/526) Antibody detects endogenous levels of Syk protein only when phosphorylated at Tyr525/526 of human Syk (Tyr519/520 of mouse Syk). It does not cross-react with other tyrosine-phosphorylated members of the Syk/Zap-70 tyrosine kinase family.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr525/526 of human Syk. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos cells, untreated or treated with anti-human IgM (12ug/ml, 2 minutes) and Jurkat cells treated with hydrogen peroxide (2mM, 2 minutes) using Phospho-Syk (Tyr525/526) Antibody.

Background

Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl (6,7). Tyr352 of Syk is involved in the association of PLCγ1 (8). Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (9).

  1. Cheng, A.M. and Chan, A.C. (1997) Curr. Opin. Immunol. 9, 528-533.
  2. Kurosaki, T. et al. (1997) Curr. Opin. Immunol. 9, 309-318.
  3. Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
  4. Turner, M. et al. (2000) Immunol. Today 21, 148-154.
  5. Coopman, P.J. et al. (2000) Nature 406, 742-747.
  6. Deckert, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.
  7. Rao, N. et al. (2001) EMBO J. 20, 7085-7095.
  8. Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.
  9. Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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