Product Pathways - Lymphocyte Signaling
Lck (V49) Antibody #2714
Have you tried your application using our XP® monoclonal antibodies? Try product: 2984
PhosphoSitePlus® protein, site, and accession data: Lck
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H | Endogenous | 56 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Lck (V49) Antibody detects endogenous levels of total Lck protein. This antibody does not cross-react with other src family proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val49 of human Lck. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat and BxPC3 cells using Lck (V49) Antibody.
Background
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
Lck is essential for T-lymphocyte activation and differentiation (3,4). Phosphorylation of Tyr505 in the carboxy-terminal tail of Lck downregulates its catalytic activity, while phosphorylation of Tyr394 leads to an increase in Lck activity (5).
- Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
- Molina, T.J. et al. (1992) Nature 357, 161-164.
- Straus, D.B. and Weiss, A. (1992) Cell 70, 585-593.
- Chow, L.M. et al. (1993) Nature 365, 156-160.
Application References
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Companion Products
- 2751 Phospho-Lck (Tyr505) Antibody
- 2752 Lck Antibody
- 2787 Lck (73A5) Rabbit mAb
- 2101 Phospho-Src Family (Tyr416) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
