Cell Signaling Technology

Product Pathways - Metabolism

MO25α/CAB39 (C49D8) Rabbit mAb #2716

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 39 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MO25α/CAB39 (C49D8) Rabbit mAb detects endogenous levels of total MO25α/CAB39 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human MO25α/CAB39.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using MO25α/CAB39 (C49D8) Rabbit mAb.

Background

AMPK (AMP-activated protein kinase) is a key cellular component that regulates energy homeostasis (1,2). When the AMP/ATP ratio is increased due to energy depletion, AMPK is phosphorylated at its α1 and α2 catalytic subunits and activated (3). Studies showed that the tumor suppressor LKB1 phosphorylates and activates AMPK in mammals (4,5). MO25α (mouse protein 25α), also called CAB39 (calcium binding protein 39), is a component of the LKB1 complex in vivo (5,6). MO25α/CAB39 does not directly interact with LKB1; instead, it is physically associated with STRADα (6). Together MO25α/CAB39 and STRADα help anchor LKB1 in the cytoplasm (6). MO25α/CAB39 stablizes the LKB1-STRADα complex and further increases the catalytic activity of LKB1 (6).

  1. Hardie, D.G. et al. (2003) FEBS Lett 546, 113-20.
  2. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
  3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
  4. Woods, A. et al. (2003) Curr Biol 13, 2004-8.
  5. Hawley, S.A. et al. (2003) J Biol 2, 28.
  6. Boudeau, J. et al. (2003) EMBO J 22, 5102-14.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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