Cell Signaling Technology

Product Pathways - Development

Wnt3a (C64F2) Rabbit mAb #2721

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP M (H) (R) Transfected Only 42 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Wnt3a (C64F2) Rabbit mAb detects transfected Wnt3a protein in L cells and the conditioned medium from L/Wnt3a cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu256 of human Wnt3a.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates and conditioned medium from L cells overexpressing mouse Wnt3a, compared with parental L cells, using Wnt3a (C64F2) Rabbit mAb.

Background

The Wnt family includes several secreted glycoproteins that play important roles in animal development (1). There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins (2). Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathways (3). The canonical Wnt/β-catenin pathway also requires a coreceptor from the low-density lipoprotein receptor family (4). Aberrant activation of Wnt signaling pathways is involved in several types of cancers (5).

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
  2. Moon, R.T. et al. (1997) Trends Genet. 13, 157-162.
  3. Kohn, A.D. and Moon, R.T. (2004) Cell Calcium 38, 439-446.
  4. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
  5. Giles, R.H. et al. (2003) Biochim. Biophys. Acta. 1653, 1-24.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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