Cell Signaling Technology

Product Pathways - Protein Folding/Stability

Hip Antibody #2723

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 48 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Hip Antibody detects endogenous levels of total Hip protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Hip. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Hip Antibody.

Background

Hip (HSP70-interacting protein), also known as ST13 (suppression of tumorigenicity protein 13), is one of several co-chaperones that regulate activities of the HSP70 chaperone family (1,2). The homo-oligomeric protein Hip cooperates with HSP70 in protein folding by stabilizing the ADP-bound state of HSP70. Hip directly binds to the ATPase domain of HSP70 when it is converted to the ADP-bound state by proteins of the HSP40 family (3). By collaborating with other positive co-factors such as HSP40 and Hop, or competing with negative co-factors such as Bag1, Hip may facilitate the chaperone function of HSP70 in protein folding and repair, and in controlling the activity of regulatory proteins such as steroid receptors and various regulators of proliferation or apoptosis (4-8).

  1. Prapapanich, V. et al. (1996) Mol. Endocrino.l 10, 420-431.
  2. Gebauer, M. et al. (1997) FEBS Lett. 417, 109-113.
  3. Höhfeld, J. et al. (1995) Cell 83, 589-.98.
  4. Frydman, J. and Höhfeld, J. (1997) Trends Biochem. Sci. 22, 87-92.
  5. Nollen, E.A. et al. (2001) J. Biol. Chem. 276, 4677-4682.
  6. Fan, G.H. et al. (2002) J. Biol. Chem. 277, 6590-6597.
  7. Nelson, G.M. et al. (2004) Mol. Endocrinol. 18, 1620-1630.
  8. Shi, Z.Z. et al. (2007) J. Zhejiang Univ. Sci. B. 8, 170-176.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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