Product Pathways - Protein Folding/Stability
Hip Antibody #2723
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 48 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Hip Antibody detects endogenous levels of total Hip protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to human Hip. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using Hip Antibody.
Background
Hip (HSP70-interacting protein) is one of several co-chaperones that regulate activities of the HSP70 chaperone family (1-2). The homo-oligomeric protein Hip cooperates with HSP70 in protein folding by stabilizing the ADP-bound state of HSP70. Hip directly binds to the ATPase domain of HSP70 when it is converted to the ADP-bound state by proteins of the HSP40 family (3). By collaborating with other positive co-factors such as HSP40 and Hop, or competing with negative co-factors such as Bag1, Hip may facilitate the chaperone function of HSP70 in protein folding and repair, and in controlling the activity of regulatory proteins such as steroid receptors and various regulators of proliferation or apoptosis (4-8).
- Prapapanich, V. et al. (1996) Mol. Endocrino.l 10, 420-431.
- Gebauer, M. et al. (1997) FEBS Lett. 417, 109-113.
- Hohfeld, J. et al. (1995) Cell 83, 589-.98.
- Frydman, J. and Hohfeld, J. (1997) Trends Biochem. Sci. 22, 87-92.
- Nollen, E.A. et al. (2001) J. Biol. Chem. 276, 4677-4682.
- Fan, G.H. et al. (2002) J. Biol. Chem. 277, 6590-6597.
- Nelson, G.M. et al. (2004) Mol. Endocrinol. 18, 1620-1630.
- Shi, Z.Z. et al. (2007) J. Zhejiang Univ. Sci. B. 8, 170-176.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
