Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

SHIP1 (P290) Antibody #2726

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC F H Endogenous 145 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SHIP1 (P290) Antibody detects endogenous levels of total SHIP1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro290 of human SHIP1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of total cell lysate from RL cells, using SHIP1 (P290) Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of RL cells, using SHIP1 (P290) Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of THP-1 (left) and Jurkat cells (right), using SHIP1 (P290) Antibody (red) and CD3 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6).

  1. Tridandapani, S. et al. (1997) Mol Cell Biol 17, 4305-11.
  2. Liu, L. et al. (1997) J Biol Chem 272, 8983-8.
  3. Malbec, O. et al. (2001) J Biol Chem 276, 30381-91.
  4. Carver, D.J. et al. (2000) Blood 96, 1449-56.
  5. Scharenberg, A.M. et al. (1998) EMBO J 17, 1961-72.
  6. Sattler, M. et al. (2001) J Biol Chem 276, 2451-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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