Product Pathways - Related Products
Normal Rabbit IgG #2729
| Applications | Reactivity | Source |
|---|---|---|
| ChIP | Rabbit |
Applications Key:
ChIP=Chromatin IP
Reactivity Key:
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Source / Purification
The purified antibody is not directed against any known antigen. It was isolated from naive rabbit sera and prepared by Protein A chromatography.
Chromatin IP
Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and the indicated antibodies. Purified DNA was analyzed by quantitative Real-Time PCR, using primers specific for the RPL30 gene, the MYOD gene or the heterochromatic α satellite repeat element. The relative abundance of each DNA sequence enriched by Normal Rabbit IgG #2729 (red) is compared to the amount of the same DNA sequence enriched by the protein-specific immunoprecipitations.
Chromatin IP
Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and either Histone H3 Antibody #2650 (lane 2), Rpb1 CTD (4H8) Mouse mAb #2629 (lane 3), Di-Methyl Histone H3 (Lys9) Antibody #9753 (lane 4) or Normal Rabbit IgG #2729 (lane 5). Purified DNA was analyzed by standard PCR methods using primers specific for the RPL30 gene, the MYOD gene or the heterochromatic α satellite repeat element. PCR products were observed for each primer set in the input sample (lane 1) and various protein-specific immunoprecipitations but no PCR products were observed with immunoprecipitation using Normal Rabbit IgG #2729 (lane 5).
Description
Normal Rabbit IgG is an unconjugated rabbit polyclonal antibody that is routinely used as a non-specific IgG control in chromatin immunoprecipitation using our SimpleChIP™ Enzymatic Chromatin IP Kits #9002 and #9003.
Background
Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
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Companion Products
- 9002 SimpleChIP™ Enzymatic Chromatin IP Kit (Agarose Beads)
- 9003 SimpleChIP™ Enzymatic Chromatin IP Kit (Magnetic Beads)
- 9006 ChIP-Grade Protein G Magnetic Beads
- 9007 ChIP-Grade Protein G Agarose Beads
- 9725 Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb
- 9726 Di-Methyl-Histone H3 (Lys4) Antibody
- 9751 Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb
- 9753 Di-Methyl-Histone H3 (Lys9) Antibody
- 4069 Pan-Methyl-Histone H3 (Lys9) Antibody
- 9756 Tri-Methyl-Histone H3 (Lys27) Antibody
- 9649 Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb
- 9677 Acetyl-Histone H3 (Lys9/Lys14) Antibody
- 2629 Rpb1 CTD (4H8) Mouse mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
